Journal: Frontiers in Oncology

Article Title: Effects of SOX10 on the proliferative, invasive, migratory, and epithelial–mesenchymal transition abilities of triple-negative breast cancer cells

doi: 10.3389/fonc.2026.1769833

Figure Lengend Snippet: Endogenous expression of SOX10 in four TNBC cell lines. (A) Relative mRNA expression of SOX10 detected by qRT-PCR. (B) Protein expression of SOX10 detected by Western blot analysis. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using one-way ANOVA with Tukey’s post-hoc test. * P < 0.05, ** P < 0.01 vs. MDA-MB-453 cell line.

Article Snippet: Four types of TNBC cell lines (HCC1937, MDA-MB-231, MDA-MB-468, and MDA-MB-453) were used in this study and purchased from ATCC (United States).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Frontiers in Oncology

Article Title: Effects of SOX10 on the proliferative, invasive, migratory, and epithelial–mesenchymal transition abilities of triple-negative breast cancer cells

doi: 10.3389/fonc.2026.1769833

Figure Lengend Snippet: Effect of SOX10 knockdown and overexpression on the proliferation of TNBC cells. Cell proliferation was assessed at 24, 48, and 72 h post-transfection, with six technical replicate wells per group at each time point and three independent biological replicates (n = 3). Data are presented as mean ± SD. (A) Proliferation curve of MDA-MB-453 cells. (B) Proliferation curve of HCC1937 cells. Statistical analysis was performed using two-way ANOVA with Tukey’s post-hoc test for multiple comparisons at each time point. siNC vs. siSOX10, * P < 0.05, ** P < 0.01; NC vs. SOX10-OE, # P < 0.05, ## P < 0.01.

Article Snippet: Four types of TNBC cell lines (HCC1937, MDA-MB-231, MDA-MB-468, and MDA-MB-453) were used in this study and purchased from ATCC (United States).

Techniques: Knockdown, Over Expression, Transfection

Journal: Frontiers in Oncology

Article Title: Effects of SOX10 on the proliferative, invasive, migratory, and epithelial–mesenchymal transition abilities of triple-negative breast cancer cells

doi: 10.3389/fonc.2026.1769833

Figure Lengend Snippet: Effect of SOX10 knockdown and overexpression on EMT-related protein expression in TNBC cells. Protein expression of E-cadherin, N-cadherin, and Vimentin was detected by Western blot analysis, with band gray values normalized to the internal reference GAPDH. (A) Protein expression and quantification in MDA-MB-453 cells. (B) Protein expression and quantification in HCC1937 cells. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using unpaired two-tailed Student’s t-test for two-group comparisons. siSOX10 vs. siNC, ** P < 0.01; SOX10-OE vs. NC, ## P < 0.01.

Article Snippet: Four types of TNBC cell lines (HCC1937, MDA-MB-231, MDA-MB-468, and MDA-MB-453) were used in this study and purchased from ATCC (United States).

Techniques: Knockdown, Over Expression, Expressing, Western Blot, Two Tailed Test

Journal: Frontiers in Oncology

Article Title: Effects of SOX10 on the proliferative, invasive, migratory, and epithelial–mesenchymal transition abilities of triple-negative breast cancer cells

doi: 10.3389/fonc.2026.1769833

Figure Lengend Snippet: Effect of SOX10 knockdown and overexpression on classical Wnt/β-catenin pathway-related protein expression in TNBC cell lines. Protein expression of C-myc, CyclinD1, WNT1, phospho-GSK-3β (Ser9), and nuclear β-catenin was detected by Western blot analysis, with band gray values normalized to GAPDH (total protein) or Lamin B1 (nuclear protein, for β-catenin). (A) Protein expression and quantification in MDA-MB-453 cells. (B) Protein expression and quantification in HCC1937 cells. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using unpaired two-tailed Student’s t-test for two-group comparisons. siSOX10 vs. siNC, ** P < 0.01; SOX10-OEvs. NC, ## P < 0.01. && indicates P<0.01; $$ indicates P<0.01 for statistical comparisons.

Article Snippet: Four types of TNBC cell lines (HCC1937, MDA-MB-231, MDA-MB-468, and MDA-MB-453) were used in this study and purchased from ATCC (United States).

Techniques: Knockdown, Over Expression, Expressing, Western Blot, Two Tailed Test